C-Jun was determined by Western blot using an antiphospho-CaMKII, phospho-PDGFR, phospho-JNK, or phospho-c-Jun antibody used at a dilution of 1:1000 in TTBS. Membranes were washed with TTBS four times for 5 min each, incubated with a 1:2000 dilution of antirabbit horseradish peroxidase antibody for 1 h. The membrane was extensively washed with TTBS. The immunoreactive bands were detected by UVP Bi